Detection of Biofilm Formation in Classical and Hypervirulent Klebsiella pneumoniae

Klebsiella pneumoniae has considered as a relevant healthcare-associated pathogen, its risk of infections is increasing in the presence medical devices. K. known for ability to form biofilm on biotic and abiotic surfaces. Biofilm assists bacterial protection from host immune responses antibiotics. Hypervirulent (hvKp) emerges new pathotype, which first appeared Asian Pacific Rim but spread globally. Thus, this study aimed investigate including hvKp potential isolates biofilm. One hundred were collected different hospitals Baghdad city. These identified by phenotypic characterization selective agar plates, biochemical tests, VITEK II, molecular identification. formation was tested these two methods, congo red Tissue Culture Plate method. In method, 33% producer (63%) can TCP method divided as: 14% strong, 15 moderate, 34% weak, 37% non-biofilm producer. The showed variable classical pneumoniae.